Recombinant Vaccinia virus (VACV) (strain Copenhagen) A27L Protein, His Tag_天津卡梅德科技发展有限公司

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Recombinant Vaccinia virus (VACV) (strain Copenhagen) A27L Protein, His Tag

Recombinant Vaccinia virus (VACV) (strain Copenhagen) A27L Protein, His Tag

产品编号

MOP2318

别名

VACV, VACV A27L

规格

100ug

DATASHEET MSDS

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产品介绍

Alias	VACV, VACV A27L
Catalog Number	MOP2318
Product Description	A DNA sequence encoding the Recombinant Vaccinia virus (VACV) (strain Copenhagen) A27L Protein (P20535) (Met1-Glu110) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	VACV
Species	VACV
Host	E. coli
Size	100ug
Purity	>90% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per ug protein as determined by the LAL method.
Formulation	PBS, 5%-8% trehalose, mannitol, and 0.01% Tween 80, pH 7.4.
Background	Vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. It has a linear, double-stranded DNA genome approximately 190 kbp in length, which encodes approximately 250 genes. The dimensions of the virion are roughly 360 × 270 × 250 nm, with a mass of approximately 5–10 fg. The vaccinia virus is the source of the modern smallpox vaccine, which the World Health Organization (WHO) used to eradicate smallpox in a global vaccination campaign in 1958–1977. Although smallpox no longer exists in the wild, vaccinia virus is still studied widely by scientists as a tool for gene therapy and genetic engineering.
Predicted N Terminus	Met
Storage Condition	The lyophilized protein can be stored at -20 ℃. After re-solubilization, the protein can be stored at -20℃ to 40℃ for one month without detectable loss of activity. The protein product is stable for 12 months from the date of receipt when stored at -80℃.
Shipping Condition	Ice packs.

FAQ

Q1-A27L蛋白在痘病毒中的功能是什么？

A27L是痘病毒的膜融合蛋白，介导病毒与宿主细胞的结合和内吞，是疫苗开发的潜在靶点。
Q2-His标签是否影响蛋白的天然构象？

标签位于C端，经圆二色谱（CD）和细胞融合实验验证，不影响蛋白功能。
Q3-能否用于痘病毒中和抗体筛选？

可以，结合假病毒系统或空斑减少中和试验（PRNT），评估抗体对痘病毒的中和能力。
Q4-储存条件有何要求？

建议-80℃分装保存，避免反复冻融（冻融次数≤3次），短期使用可暂存于-20℃。
Q5-是否支持大规模生产？

支持，采用昆虫细胞表达系统，可提供克级GMP标准蛋白。

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