Recombinant Monkeypox Virus (MPXV) A33R Protein, His & GST Tag_天津卡梅德科技发展有限公司

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Recombinant Monkeypox Virus (MPXV) A33R Protein, His & GST Tag

Recombinant Monkeypox Virus (MPXV) A33R Protein, His & GST Tag

产品编号

MOP2305

别名

MPXV, MPXV A33R

规格

100ug

DATASHEET MSDS

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产品介绍

Alias	MPXV, MPXV A33R
Catalog Number	MOP2305
Product Description	A DNA sequence encoding the Recombinant Monkeypox Virus (MPXV) Protein A33R was fused with the N-terminal polyhistidine tag followed by a GST tag.
Molecular Name	MPXV
Species	MPXV
Host	E. coli
Size	100ug
Purity	>85% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per ug protein as determined by the LAL method.
Formulation	50mM Tris, 150mM NaCl, 1mM EDTA, 10% Glycerol, 5%-8% trehalose, mannitol, and 0.01% Tween 80, pH 8.
Background	The monkeypox virus (MPV, MPXV, or hMPXV), also called the mpox virus, is a species of double-stranded DNA virus that causes mpox in humans and other mammals. the monkeypox virus is a zoonotic virus belonging to the orthopoxvirus genus, making it closely related to the variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. MPV is oval-shaped with a lipoprotein outer membrane. The genome is approximately 190 kb. The smallpox and monkeypox viruses are both orthopoxviruses, and the smallpox vaccine is effective against mpox if given within 3–5 years before contracting the disease. The clinical presentation of mpox is similar to smallpox, but with a milder rash and lower mortality rate. The virus is transmissible between animals and humans by direct contact to the lesions or bodily fluids. The virus was given the name monkeypox virus after being isolated from monkeys, but most of the carriers of this virus are rodents.
Predicted N Terminus	Met
Storage Condition	The lyophilized protein can be stored at -20 ℃. After re-solubilization, the protein can be stored at -20℃ to 40℃ for one month without detectable loss of activity. The protein product is stable for 12 months from the date of receipt when stored at -80℃.
Shipping Condition	Ice packs.

FAQ

Q1-A33R蛋白在猴痘病毒感染中的作用是什么？

A33R是猴痘病毒的包膜蛋白，参与病毒粒子在细胞间的传播，是中和抗体识别和疫苗开发的关键靶点。
Q2-His和GST双标签的作用是什么？如何去除？

His标签用于镍柱亲和层析纯化，GST标签增强蛋白可溶性；GST可通过PreScission蛋白酶切割去除（需在订购时指定）。
Q3-该蛋白能否用于猴痘抗体检测？

是的，作为ELISA或免疫印迹（Western Blot）的包被抗原，特异性检测患者血清中的IgG/IgM抗体。
Q4-蛋白纯度如何验证？

通过SDS-PAGE（纯度≥95%）和质谱分析，确保无宿主蛋白或降解产物残留。
Q5-是否支持动物免疫实验？

支持，提供佐剂（如铝佐剂或MF59）配伍建议，已用于小鼠模型诱导中和抗体。

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