ZIKV (strain Zika SPH2016) Envelope GP E Protein, Fc Tag_天津卡梅德科技发展有限公司

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ZIKV (strain Zika SPH2016) Envelope GP E Protein, Fc Tag

ZIKV (strain Zika SPH2016) Envelope GP E Protein, Fc Tag

产品编号

KMP4919

别名

寨卡病毒包膜蛋白E, ZIKV Envelope GP E, Envelope, ZIKV Envelope GP E Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	寨卡病毒包膜蛋白E, ZIKV Envelope GP E, Envelope, ZIKV Envelope GP E Protein
Catalog Number	KMP4919
Product Description	The ZIKV (strain Zika SPH2016) Envelope GP E Protein(KMP4919) is produced in HEK293 Cells. A DNA sequence encoding the Zika virus (strain Zika SPH2016) E_stem (Stem/anchor domain of flavivirus envelope glycoprotein E) (ALU33341.1) (Gly698-Ala794) was expressed with the Fc region of human IgG1 at the C-terminus.
Molecular Name	ZIKV Envelope GP E
Species	ZIKV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-GP E蛋白的命名与功能有何关联？

GP E即包膜糖蛋白（Envelope Glycoprotein），与M蛋白共同形成病毒表面刺突，介导宿主细胞入侵。
Q2-2016株与2015株的差异是什么？

2016株可能包含抗原漂移突变，需测序比对确认，但其GP E核心功能（受体结合）高度保守。
Q3-能否用于快速诊断试纸条开发？

是的，作为抗原可检测患者血清中的IgM/IgG，但需与登革病毒抗原区分以避免交叉反应。
Q4-如何验证糖基化修饰？

质谱鉴定N-糖基化位点（如Asn-154），或PNGase F酶切后观察SDS-PAGE迁移率变化。
Q5-运输过程中如何维持活性？

干冰运输，到货后立即-80℃保存；避免高温暴露导致蛋白变性。

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