WNV (lineage 1, strain NY99) Envelope Protein, His Tag_天津卡梅德科技发展有限公司

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WNV (lineage 1, strain NY99) Envelope Protein, His Tag

WNV (lineage 1, strain NY99) Envelope Protein, His Tag

产品编号

KMP4915

别名

西尼罗河病毒包膜蛋白, WNV Envelope, E Protein, WNV Envelope Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	西尼罗河病毒包膜蛋白, WNV Envelope, E Protein, WNV Envelope Protein
Catalog Number	KMP4915
Product Description	The WNV (lineage 1, strain NY99) Envelope Protein(KMP4915) is produced in Yeast. A DNA sequence encoding the domain III of the West Nile virus (WNV)((lineage 1, strain NY99) envelope glycoprotein (E) (ABA62343.1) (Gln586-Ala705) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	WNV Envelope
Species	WNV
Host	Yeast
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-包膜蛋白的功能是什么？

包膜蛋白介导病毒与宿主细胞（如神经元）结合，并通过膜融合促进入侵，是中和抗体的主要靶标。
Q2-NY99株的流行病学意义是什么？

该株是1999年北美西尼罗病毒暴发的病原体，研究其包膜蛋白有助于疫苗和诊断试剂开发。
Q3-如何验证蛋白的受体结合活性？

通过ELISA检测与DC-SIGN或整合素等宿主受体的结合，或假病毒中和实验。
Q4-是否形成二聚体结构？

包膜蛋白以二聚体形式存在，通过非还原SDS-PAGE或SEC验证其寡聚状态。
Q5-储存条件如何优化？

-80℃保存，溶解后分装使用；缓冲液中含0.01% Tween-20防止聚集。

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