SARS (isolate Tor2) Spike RBD Protein, His Tag, Avi Tag_天津卡梅德科技发展有限公司

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SARS (isolate Tor2) Spike RBD Protein, His Tag, Avi Tag

SARS (isolate Tor2) Spike RBD Protein, His Tag, Avi Tag

产品编号

KMP4908

别名

冠状病毒刺突蛋白受体结构域, SARS Spike RBD, S Protein, SARS Spike RBD Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	冠状病毒刺突蛋白受体结构域, SARS Spike RBD, S Protein, SARS Spike RBD Protein
Catalog Number	KMP4908
Product Description	The SARS (Isolate Tor2) Spike RBD Protein(KMP4908) is produced in HEK293 Cells. A DNA sequence encoding the SARS-CoV (isolate Tor2) spike RBD (NP_828851.1) (Arg306-Phe527) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Molecular Name	SARS Spike RBD
Species	SARS
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	50mM Tris, 250mM NaCl, 1mM EDTA, 50% Glycerol, pH7.0
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-RBD蛋白的功能和意义是什么？

RBD（受体结合域）是SARS-CoV刺突蛋白的关键区域，直接结合宿主ACE2受体，介导病毒入侵，是中和抗体和疫苗研发的核心靶点。
Q2-His和Avi双标签的作用是什么？

His标签用于镍柱纯化，Avi标签允许位点特异性生物素化，便于基于链霉亲和素的检测（如ELISA、SPR或单颗粒分析）。
Q3-如何验证RBD与ACE2的结合活性？

通过表面等离子共振（SPR）或ELISA检测其与重组ACE2蛋白的亲和力（KD值通常为nM级）。
Q4-能否用于mRNA疫苗抗原设计？

是的，RBD是mRNA疫苗（如Moderna早期候选疫苗）的常用抗原，可诱导高效中和抗体。
Q5-储存条件如何优化？

-80℃保存，避免反复冻融；生物素化后4℃稳定1周，长期需添加50%甘油防冻。

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