SARS (strain WH20) Plpro Protein, His Tag_天津卡梅德科技发展有限公司

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SARS (strain WH20) Plpro Protein, His Tag

SARS (strain WH20) Plpro Protein, His Tag

产品编号

KMP4905

别名

冠状病毒木瓜蛋白酶样蛋白酶, SARS Plpro, Papain-like Protease, SARS Plpro Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	冠状病毒木瓜蛋白酶样蛋白酶, SARS Plpro, Papain-like Protease, SARS Plpro Protein
Catalog Number	KMP4905
Product Description	The SARS (strain WH20) Plpro Protein(KMP4905) is produced in E. coli. A DNA sequence encoding the SARS-CoV (strain WH20) papain-like protease (PLpro) (AAX16193.1) (Glu1541-Tyr1859) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	SARS Plpro
Species	SARS
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-Plpro蛋白酶的功能是什么？

木瓜蛋白酶样蛋白酶（Plpro）切割病毒多蛋白（pp1a/pp1ab）释放非结构蛋白，并具有去泛素化（DUB）活性，抑制宿主先天免疫应答。
Q2-如何检测Plpro的酶活性？

使用荧光底物（如Z-RLRGG-AMC）检测切割效率，或通过Western Blot验证去泛素化功能。
Q3-能否用于抗病毒药物筛选？

是的，Plpro是药物开发的重要靶点，可筛选小分子抑制剂（如GRL-0617类似物）。
Q4-His标签是否影响酶活性？

标签位于N或C末端（需确认设计），经验证不影响催化活性或底物结合。
Q5-储存条件有何建议？

添加1 mM DTT和10%甘油，-80℃保存；避免反复冻融，分装后使用以维持酶活性。

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