SARS S2 Protein
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产品编号
KMP4903
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别名
冠状病毒刺突蛋白S2, SARS S2, Spike2 Protein, SARS S2 Protein
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规格
- 50ug
- 100ug
- 200ug
Alias | 冠状病毒刺突蛋白S2, SARS S2, Spike2 Protein, SARS S2 Protein |
Catalog Number | KMP4903 |
Product Description | The SARS S2 Protein(KMP4903) is produced in Insect Cells. A DNA sequence encoding the SARS-CoV spike S2 (NP_828851.1) (Ser668-Pro1195) was expressed with a C-terminal polyhistidine tag. |
Molecular Name | SARS S2 |
Species | SARS |
Host | Insect Cells |
Size | 50ug, 100ug, 200ug |
Purity | >95% as determined by SDS-PAGE |
Purification | Affinity purification |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Formulation | 50mM Tris, 500mM NaCl |
Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
S2包含融合肽(FP)、七肽重复区1/2(HR1/HR2),介导病毒与宿主细胞膜融合,是泛冠状病毒抑制剂(如EK1多肽)的靶点。
需在酸性pH条件下激活,通过脂质体融合实验或细胞-细胞融合模型验证其功能。
是的,HR1-HR2复合物可形成六螺旋束(6-HB),是X射线晶体学或冷冻电镜的热门靶点。
通过离子交换层析(如SP柱)或疏水相互作用层析(HIC)纯化,依赖蛋白表面电荷和疏水性。
是的,S2区域在冠状病毒中高度保守,可筛选靶向融合过程的抑制剂。