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SARS S1 Protein, mFc Tag

SARS S1 Protein, mFc Tag

产品编号

KMP4898

别名

冠状病毒刺突蛋白S1, SARS S1, Spike1 Protein, SARS S1 Protein

规格
  • 50ug
  • 100ug
  • 200ug
产品介绍
Alias冠状病毒刺突蛋白S1, SARS S1, Spike1 Protein, SARS S1 Protein
Catalog NumberKMP4898
Product DescriptionThe SARS S1 Protein(KMP4898) is produced in HEK293 Cells. A DNA sequence encoding the SARS-CoV (isolate:WH20) spike (S1 Subunit)(AAX16192.1) (Met1-Arg667) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Molecular NameSARS S1
SpeciesSARS
HostHEK293 Cells
Size50ug, 100ug, 200ug
Purity>90% as determined by SDS-PAGE
PurificationAffinity purification
Endotoxin<1.0 EU/ug determined by the LAL method
Formulation50mM Tris, 500mM NaCl, pH8.0
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
FAQ
  • Q1-S1与全长Spike蛋白的应用区别是什么?

    S1聚焦受体结合域(RBD)研究,适用于抗体中和及诊断,而全长Spike更适用于膜融合或疫苗开发。

  • Q2-mFc标签是否干扰ACE2结合?

    标签位于C端,经SPR验证不影响与ACE2受体的结合活性(KD≈1 nM)。

  • Q3-能否用于ELISA检测患者血清抗体?

    是的,作为包被抗原时灵敏度高,可检测SARS康复者血清中的IgG/IgM。

  • Q4-是否提供糖基化位点分析?

    可提供质谱鉴定的N-糖基化图谱(如Asn-330、Asn-357),需额外订购。

  • Q5-运输过程中如何维持活性?

    液氮速冻后干冰运输,到货后立即-80℃保存,避免温度波动导致变性。

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