RSV (subtype A, strain Long) GP Protein(ECD), His Tag
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产品编号
KMP4891
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别名
人呼吸道合胞病毒包膜糖蛋白, RSV GP, Glycoprotein, RSV GP Protein
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规格
- 50ug
- 100ug
- 200ug
产品介绍
| Alias | 人呼吸道合胞病毒包膜糖蛋白, RSV GP, Glycoprotein, RSV GP Protein |
| Catalog Number | KMP4891 |
| Product Description | The RSV (subtype A, strain Long) GP Protein(KMP4891) is produced in HEK293 Cells. A DNA sequence encoding the human respiratory syncytial virus (RSV) (subtype A, strain Long) glycoprotein G (P20895-1) (His67-Gln298) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | RSV GP |
| Species | RSV |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0 |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
FAQ
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Q1-RSV G蛋白的生物学功能是什么？
G蛋白（附着糖蛋白）介导病毒与宿主细胞表面的硫酸乙酰肝素蛋白聚糖（HSPG）结合，是病毒初始黏附的关键蛋白，也参与免疫调节（如CX3C趋化因子模拟）。
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Q2-ECD（胞外域）的设计有何优势？
去除跨膜区和胞内域，提高可溶性，保留受体结合功能，适用于抗体中和研究或ELISA抗原开发。
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Q3-该蛋白能否用于疫苗研发？
G蛋白诱导的抗体中和能力较弱，但可与F蛋白联用增强免疫应答，目前更多用于诊断或机制研究。
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Q4-如何验证G蛋白的糖基化修饰？
通过PNGase F酶切后SDS-PAGE分析迁移率变化，或质谱鉴定N-糖基化位点（如Thr-300）。
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Q5-His标签是否影响蛋白活性？
标签位于C末端，经ELISA和细胞结合实验验证不影响其与宿主受体的相互作用。
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