H3N2 (A/Darwin/9/2021) HA Trimer Protein, His Tag_天津卡梅德科技发展有限公司

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H3N2 (A/Darwin/9/2021) HA Trimer Protein, His Tag

H3N2 (A/Darwin/9/2021) HA Trimer Protein, His Tag

产品编号

KMP4773

别名

甲型流感病毒H3N2血凝素三聚体蛋白, H3N2 HA Trimer, Hemagglutinin trimer Protein, H3N2 HA Trimer Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H3N2血凝素三聚体蛋白, H3N2 HA Trimer, Hemagglutinin trimer Protein, H3N2 HA Trimer Protein
Catalog Number	KMP4773
Product Description	The H3N2 (A/Darwin/9/2021) HA Trimer Protein(KMP4773) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus ((A/Darwin/9/2021) (H3N2)) hemagglutinin (translated amino acids of EPI1859996) (Met1-Val521), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
Molecular Name	H3N2 HA Trimer
Species	H3N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA三聚体蛋白相比单体有何优势？

三聚体结构模拟病毒表面天然构象，更易诱导中和抗体，是疫苗研发的理想抗原形式。
Q2-达尔文2021株HA蛋白的流行病学意义是什么？

该株属于季节性H3N2分支，其HA抗原漂移可能导致疫苗效力下降，研究其结构可优化疫苗匹配性。
Q3-如何验证三聚体的正确组装？

通过凝胶过滤层析（SEC-HPLC）和负染电镜确认其以均一三聚体形式存在。
Q4-该蛋白适用于哪些研究？

可用于中和抗体评价、疫苗免疫原性测试及受体结合动力学分析。
Q5-储存和运输条件有何要求？

液氮速冻后-80℃保存，干冰运输，避免反复冻融以维持三聚体稳定性。

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