H1N2 (A/Swine/Indiana/P12439/00) NA Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H1N2 (A/Swine/Indiana/P12439/00) NA Protein, His Tag

H1N2 (A/Swine/Indiana/P12439/00) NA Protein, His Tag

产品编号

KMP4745

别名

甲型流感病毒H1N2神经氨酸酶, H1N2 NA, Neuraminidase Protein, H1N2 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H1N2神经氨酸酶, H1N2 NA, Neuraminidase Protein, H1N2 NA Protein
Catalog Number	KMP4745
Product Description	The H1N2 (A/Swine/Indiana/P12439/00) NA Protein(KMP4745) is produced in HEK293 Cells. A DNA sequence encoding the influenza A virus (A/Swine/Indiana/P12439/00 (H1N2)) neuraminidase (AAL87886) (His36-Ile469), termed as NA, was fused with a N-terminal polyhistidine tag.
Molecular Name	H1N2 NA
Species	H1N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NA蛋白在流感病毒生命周期中的功能是什么？

NA（神经氨酸酶）通过切割宿主细胞表面唾液酸残基，促进病毒释放，是抗病毒药物（如奥司他韦）的作用靶点。
Q2-印第安纳猪源H1N2毒株的研究价值是什么？

该毒株反映了北美猪群中H1N2的流行特征，其NA蛋白研究可为区域性流感防控提供依据。
Q3-His标签是否影响NA的酶活性？

活性检测表明，His标签不影响NA切割MUNANA底物的能力，酶活单位（NAU）符合标准。
Q4-能否用于耐药性突变研究？

是的，可引入已知耐药位点（如H274Y），评估其对NA抑制剂的敏感性变化。
Q5-如何检测NA蛋白的活性？

使用荧光底物MUNANA，通过检测荧光强度（Ex/Em=365/450 nm）定量酶活。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0