H5N6 (A/duck/Jiangxi/95/2014) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H5N6 (A/duck/Jiangxi/95/2014) HA1 Protein, His Tag

H5N6 (A/duck/Jiangxi/95/2014) HA1 Protein, His Tag

产品编号

KMP4620

别名

甲型流感病毒H5N6血凝素蛋白1, H5N6 HA1, Hemagglutinin Protein, H5N6 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N6血凝素蛋白1, H5N6 HA1, Hemagglutinin Protein, H5N6 HA1 Protein
Catalog Number	KMP4620
Product Description	The H5N6 (A/duck/Jiangxi/95/2014) HA1 Protein(KMP4620) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/duck/Jiangxi/95/2014(H5N6)) hemagglutinin (translated amino acids of EPI530054) (Met1-Arg345), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H5N6 HA1
Species	H5N6
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1与全长HA的抗原性是否一致？

HA1包含主要中和表位，与全长HA的抗体结合特性高度一致，适合替代全长蛋白用于ELISA检测。
Q2-能否用于快速诊断试纸条开发？

是的，可标记为检测线抗原，配对抗H5单克隆抗体实现双抗体夹心法检测。
Q3-是否提供热稳定性数据？

37℃保存1周后活性保留>70%，建议短期使用冷藏，长期保存需-80℃。
Q4-该蛋白的糖基化是否影响抗体识别？

糖基化可能遮蔽部分表位，可通过去糖基化处理（如PNGase F）对比实验验证影响。
Q5-是否支持抗体亲和力成熟研究？

是的，可作为抗原用于噬菌体展示文库筛选，优化抗体亲和力及特异性。

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