H5N6 (A/duck/Jiangxi/95/2014) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H5N6 (A/duck/Jiangxi/95/2014) HA Protein, His Tag

H5N6 (A/duck/Jiangxi/95/2014) HA Protein, His Tag

产品编号

KMP4619

别名

甲型流感病毒H5N6血凝素蛋白, H5N6 HA, Hemagglutinin Protein, H5N6 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N6血凝素蛋白, H5N6 HA, Hemagglutinin Protein, H5N6 HA Protein
Catalog Number	KMP4619
Product Description	The H5N6 (A/duck/Jiangxi/95/2014) HA Protein(KMP4619) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/duck/Jiangxi/95/2014(H5N6)) hemagglutinin (translated amino acids of EPI530054) (Met1-Gln530), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H5N6 HA
Species	H5N6
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-H5N6 HA蛋白的公共卫生风险是什么？

H5N6是高致病性禽流感病毒，可感染人类且致死率高，研究其HA有助于疫苗和诊断试剂开发。
Q2-该蛋白是否含有多碱性切割位点？

是的，H5亚型HA的切割位点含多个碱性氨基酸（如RRKR），提示高致病性特征。
Q3-能否用于假病毒中和实验？

是的，与NA蛋白共表达可包装假病毒，评估血清中和抗体效价（需生物安全审批）。
Q4-是否验证与禽/人受体结合能力？

经糖结合实验证实，其同时结合α-2,3和α-2,6唾液酸受体，可能增强跨物种传播潜力。
Q5-如何确保蛋白无核酸污染？

纯化过程中使用Benzonase处理，核酸残留<0.1 ng/μg（Qubit荧光定量检测）。

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