H5N1 (A/bar-headed goose/Qinghai/1A/2005) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H5N1 (A/bar-headed goose/Qinghai/1A/2005) HA1 Protein, His Tag

H5N1 (A/bar-headed goose/Qinghai/1A/2005) HA1 Protein, His Tag

产品编号

KMP4516

别名

甲型流感病毒H5N1血凝素蛋白1, H5N1 HA1, Hemagglutinin Protein, H5N1 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N1血凝素蛋白1, H5N1 HA1, Hemagglutinin Protein, H5N1 HA1 Protein
Catalog Number	KMP4516
Product Description	The H5N1 (A/bar-headed goose/Qinghai/1A/2005) HA1 Protein(KMP4516) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/bar-headed goose/Qinghai/1A/2005(H5N1)) hemagglutinin (ABF93441.1) (Met1-Arg342), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H5N1 HA1
Species	H5N1
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白是否具有高致病性禽流感（HPAI）特征？

是的，含有多碱性切割位点，需在BSL-3实验室操作活病毒相关实验。
Q2-能否用于中和抗体检测？

可以，结合假病毒系统或微量中和实验，评估血清抗体对H5N1的中和能力。
Q3-如何控制内毒素水平？

采用无内毒素表达系统（如昆虫细胞），并通过LAL检测确保内毒素＜0.1 EU/μg。
Q4-是否支持HA三聚体的定制？

支持，通过分子伴侣共表达或结构稳定突变（如T4 fibritin标签），增强三聚体形成。
Q5-运输和储存有何特殊要求？

需全程-80℃保存，运输使用干冰，附带生物安全运输证明。

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