H3N2 (A/Hong Kong/1/1968) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H3N2 (A/Hong Kong/1/1968) HA1 Protein, His Tag

H3N2 (A/Hong Kong/1/1968) HA1 Protein, His Tag

产品编号

KMP4514

别名

甲型流感病毒H3N2血凝素蛋白1, H3N2 HA1, Hemagglutinin Protein, H3N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H3N2血凝素蛋白1, H3N2 HA1, Hemagglutinin Protein, H3N2 HA1 Protein
Catalog Number	KMP4514
Product Description	The H3N2 (A/Hong Kong/1/1968) HA1 Protein(KMP4514) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/Hong Kong/1/1968(H3N2) hemagglutinin (AAK51718.1) (Met1-Arg345), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H3N2 HA1
Species	H3N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1亚基是否保留血凝活性？

是的，通过血凝试验（HAU）验证，每μg蛋白可凝集≥1×10^6鸡红细胞。
Q2-能否用于季节性流感疫苗的效力评价？

可以，作为参考抗原用于疫苗免疫原性检测或中和抗体效价测定。
Q3-如何验证蛋白的糖基化修饰？

采用PNGase F消化结合质谱分析，确认N-糖基化位点与天然病毒一致。
Q4-是否支持HA1与HA2的共表达？

支持，可共表达HA1和HA2亚基，模拟天然HA的膜融合活性。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后避免反复冻融。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0