H7N9 (A/Anhui/1/2013) NA Protein, His Tag_天津卡梅德科技发展有限公司

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H7N9 (A/Anhui/1/2013) NA Protein, His Tag

H7N9 (A/Anhui/1/2013) NA Protein, His Tag

产品编号

KMP4512

别名

甲型流感病毒H7N9神经氨酸酶, H7N9 NA, Neuraminidase Protein, H7N9 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H7N9神经氨酸酶, H7N9 NA, Neuraminidase Protein, H7N9 NA Protein
Catalog Number	KMP4512
Product Description	The H7N9 (A/Anhui/1/2013) NA Protein(KMP4512) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) neuraminidase (EPI439509) (His36-Leu465) was expressed with an N-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Molecular Name	H7N9 NA
Species	H7N9
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该NA蛋白是否适用于H7N9疫苗研发？

是的，作为灭活疫苗或亚单位疫苗的候选抗原，诱导针对NA的特异性免疫应答。
Q2-如何控制内毒素水平？

采用无内毒素表达系统（如HEK293细胞），并通过LAL检测确保内毒素＜0.1 EU/μg。
Q3-能否用于假病毒包装？

可以，与HA蛋白共表达构建假病毒，评估抗体对病毒释放的抑制作用。
Q4-是否提供酶活性检测报告？

是的，提供基于MUNANA底物的酶活性数据（IC50值）。
Q5-运输条件有何要求？

液体需干冰运输，冻干粉可常温运输，附带温度记录仪。

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