H9N2 (A/chicken/Hong Kong/G9/1997) NA Protein, His Tag_天津卡梅德科技发展有限公司

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H9N2 (A/chicken/Hong Kong/G9/1997) NA Protein, His Tag

H9N2 (A/chicken/Hong Kong/G9/1997) NA Protein, His Tag

产品编号

KMP4506

别名

甲型流感病毒H9N2神经氨酸酶, H9N2 NA, Neuraminidase Protein, H9N2 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H9N2神经氨酸酶, H9N2 NA, Neuraminidase Protein, H9N2 NA Protein
Catalog Number	KMP4506
Product Description	The H9N2 (A/chicken/Hong Kong/G9/1997) NA Protein(KMP4506) is produced in HEK293 Cells. A DNA sequence encoding the influenza A virus (A/chicken/Hong Kong/G9/1997(H9N2)) neuraminidase (AAD49001.1) (His36-Ile469), termed as NA, was fused with a N-terminal polyhistidine tag.
Molecular Name	H9N2 NA
Species	H9N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NA蛋白在流感病毒中的功能是什么？

神经氨酸酶（NA）介导病毒从宿主细胞释放，是抗病毒药物（如奥司他韦）的靶点。
Q2-能否用于药物耐药性研究？

可以，通过酶活性实验评估NA对奥司他韦的敏感性，筛选耐药突变株。
Q3-如何验证NA的酶活性？

采用荧光底物（如MUNANA）检测其切割唾液酸的活性，计算比活性（U/mg）。
Q4-是否提供预包被ELISA板？

支持，提供预包被NA的96孔板，优化反应条件，直接用于抗体检测。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

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