H12N1 (A/mallard duck/Alberta/342/1983) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H12N1 (A/mallard duck/Alberta/342/1983) HA Protein, His Tag

H12N1 (A/mallard duck/Alberta/342/1983) HA Protein, His Tag

产品编号

KMP4505

别名

甲型流感病毒H12N1血凝素蛋白, H12N1 HA, Hemagglutinin Protein, H12N1 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H12N1血凝素蛋白, H12N1 HA, Hemagglutinin Protein, H12N1 HA Protein
Catalog Number	KMP4505
Product Description	The H12N1 (A/mallard duck/Alberta/342/1983) HA Protein(KMP4505) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/mallard duck/Alberta/342/1983(H12N1)) hemagglutinin (ABB88099.1)(Met1-Lys527), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H12N1 HA
Species	H12N1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否包含跨膜域？

不包含，仅包含胞外域（ECD），适用于可溶性表达和功能研究。
Q2-能否用于广谱中和抗体的筛选？

可以，与其他亚型（如H1、H3）HA蛋白对比，评估抗体对不同毒株的中和能力。
Q3-如何检测蛋白的天然构象？

通过ELISA验证其与构象依赖性单克隆抗体的结合能力，确保结构完整性。
Q4-是否提供冻干粉和液体两种形式？

支持，冻干粉可常温运输，液体需-80℃保存，用户可根据需求选择。
Q5-是否支持HA与CD40L等分子佐剂的融合表达？

支持，可构建融合蛋白以增强抗原呈递效率，需提供目标序列。

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