H10N3 (A/duck/Hong Kong/786/1979) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H10N3 (A/duck/Hong Kong/786/1979) HA Protein, His Tag

H10N3 (A/duck/Hong Kong/786/1979) HA Protein, His Tag

产品编号

KMP4485

别名

甲型流感病毒H10N3血凝素蛋白, H10N3 HA, Hemagglutinin Protein, H10N3 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H10N3血凝素蛋白, H10N3 HA, Hemagglutinin Protein, H10N3 HA Protein
Catalog Number	KMP4485
Product Description	The H10N3 (A/duck/Hong Kong/786/1979) HA Protein(KMP4485) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/duck/Hong Kong/786/1979(H10N3)) hemagglutinin (BAF46762.1) (Met1-Ile525) termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H10N3 HA
Species	H10N3
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, 1% Triton X-100, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否具有人畜共患病风险研究的价值？

是的，用于分析HA与人类呼吸道受体的结合能力，评估潜在跨物种传播风险。
Q2-如何验证蛋白的糖基化修饰？

通过PNGase F消化和质谱分析，确认N-糖基化位点与天然病毒一致。
Q3-能否用于单克隆抗体的开发？

可以，作为免疫原或检测抗原，用于小鼠或兔杂交瘤技术筛选中和抗体。
Q4-储存时是否需要避光？

建议避光保存于-80℃，防止光敏性降解或氧化。
Q5-是否支持HA与神经氨酸酶（NA）的共表达？

支持，可共表达H10N3 HA与NA蛋白，用于假病毒系统构建或疫苗研究。

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