H2N2 (A/Canada/720/2005) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H2N2 (A/Canada/720/2005) HA Protein, His Tag

H2N2 (A/Canada/720/2005) HA Protein, His Tag

产品编号

KMP4483

别名

甲型流感病毒H2N2血凝素蛋白, H2N2 HA, Hemagglutinin Protein, H2N2 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H2N2血凝素蛋白, H2N2 HA, Hemagglutinin Protein, H2N2 HA Protein
Catalog Number	KMP4483
Product Description	The H2N2 (A/Canada/720/2005) HA Protein(KMP4483) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/Canada/720/2005(H2N2)) hemagglutinin (AAY28987.1) (Met1-Gln525), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H2N2 HA
Species	H2N2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, 187mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, 1% TritonX-100, 6% Trehalose, 5.3% manicol, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否适用于季节性流感疫苗研发？

是的，作为H2N2亚型的候选抗原，可用于灭活疫苗或重组亚单位疫苗的免疫原性评估。
Q2-如何检测HA蛋白的受体结合特异性？

通过唾液酸受体类似物（如α-2,6连接）结合实验，验证其对人类呼吸道上皮的亲和力。
Q3-蛋白批次间一致性如何控制？

每批次均通过SDS-PAGE、HPLC和血凝活性检测，确保差异≤5%。
Q4-能否用于假病毒包装？

可以，与NA蛋白共表达构建假病毒，用于中和抗体或抗病毒药物筛选。
Q5-运输条件是什么？

液体需干冰运输，冻干粉可常温运输，附带温度记录仪。

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