H1N3 (A/duck/NZL/160/1976) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H1N3 (A/duck/NZL/160/1976) HA Protein, His Tag

H1N3 (A/duck/NZL/160/1976) HA Protein, His Tag

产品编号

KMP4482

别名

甲型流感病毒H1N3血凝素蛋白, H1N3 HA, Hemagglutinin Protein, H1N3 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H1N3血凝素蛋白, H1N3 HA, Hemagglutinin Protein, H1N3 HA Protein
Catalog Number	KMP4482
Product Description	The H1N3 (A/duck/NZL/160/1976) HA Protein(KMP4482) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/duck/NZL/160/1976 (H1N3)) hemagglutinin (ABB20429.1) (Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H1N3 HA
Species	H1N3
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白的主要应用领域是什么？

用于禽流感病毒（H1N3亚型）研究，包括疫苗开发、中和抗体筛选及跨物种传播机制分析。
Q2-His标签是否影响蛋白的天然构象？

His标签位于C端，经圆二色谱（CD）和血凝试验（HAU）验证，不影响HA的受体结合或折叠结构。
Q3-如何验证蛋白的纯度？

通过SDS-PAGE（纯度≥95%）和HPLC分析，并提供质谱鉴定报告。
Q4-储存条件对蛋白稳定性有何要求？

建议-80℃分装保存，避免反复冻融（冻融次数≤3次），短期使用可暂存于-20℃。
Q5-是否支持其他禽流感毒株的HA蛋白定制？

支持，需提供目标毒株的HA基因序列，可定制表达及功能验证服务。

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