H5N1 (A/Anhui/1/2005) NA Protein, His Tag_天津卡梅德科技发展有限公司

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H5N1 (A/Anhui/1/2005) NA Protein, His Tag

H5N1 (A/Anhui/1/2005) NA Protein, His Tag

产品编号

KMP4479

别名

甲型流感病毒H5N1神经氨酸酶, H5N1 NA, Neuraminidase Protein, H5N1 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N1神经氨酸酶, H5N1 NA, Neuraminidase Protein, H5N1 NA Protein
Catalog Number	KMP4479
Product Description	The H5N1 (A/Anhui/1/2005) NA Protein(KMP4479) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/Anhui/1/2005 (H5N1)) neuraminidase (ABU94738.1) (His36-Lys449) was expressed with a vasodilator-stimulated phosphoprotein tetramerization domain at the N-terminus and a polyhistidine tag at the C-terminus.
Molecular Name	H5N1 NA
Species	H5N1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, 1% Triton X-100, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该NA蛋白的酶学特性如何？

经MUNANA实验检测，比活性≥5000 U/mg，对奥司他韦敏感（IC50≤10 nM）。
Q2-能否用于耐药突变研究？

支持定制H274Y或N294S等耐药突变体，评估其对神经氨酸酶抑制剂的敏感性变化。
Q3-是否形成四聚体？

经非还原SDS-PAGE和SEC验证，NA以四聚体形式存在，二硫键稳定。
Q4-如何检测其与HA的功能协同性？

与HA共转染细胞，通过病毒样颗粒（VLP）释放实验评估NA对病毒出芽的促进作用。
Q5-推荐用于酶活抑制实验的浓度？

反应体系中NA浓度建议0.1-1 μg/ml，底物MUNANA使用50 μM，37°C孵育1小时。

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