H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP Protein, His Tag_天津卡梅德科技发展有限公司

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H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP Protein, His Tag

H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP Protein, His Tag

产品编号

KMP4477

别名

甲型流感病毒H1N1核蛋白, H1N1 NP, Nucleoprotein, H1N1 NP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H1N1核蛋白, H1N1 NP, Nucleoprotein, H1N1 NP Protein
Catalog Number	KMP4477
Product Description	The H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP Protein(KMP4477) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) Nucleoprotein / NP (AAM75159.1) (Met1-Asn498) was fused with a polyhistidine tag at the C-terminus.
Molecular Name	H1N1 NP
Species	H1N1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, 1% Triton X-100, 5% Trehalose, 5% Mannitol, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NP蛋白在流感病毒中的功能？

核蛋白（NP）包裹病毒RNA形成核糖核蛋白复合体（RNP），是病毒复制和转录的核心。
Q2-能否用于T细胞免疫研究？

是的，NP是主要T细胞表位来源，用于评估疫苗诱导的细胞免疫应答（如IFN-γ ELISpot）。
Q3-是否形成寡聚体？

经动态光散射（DLS）分析，NP以三聚体形式存在，与天然病毒RNP结构一致。
Q4-如何验证其RNA结合能力？

通过凝胶迁移实验（EMSA）或荧光偏振（FP）检测与病毒RNA启动子序列的结合。
Q5-推荐保存条件？

分装后-80℃保存，缓冲液含1 mM DTT和10%甘油，避免反复冻融导致聚集。

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