H9N2 (A/Hong Kong/1073/99) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H9N2 (A/Hong Kong/1073/99) HA1 Protein, His Tag

H9N2 (A/Hong Kong/1073/99) HA1 Protein, His Tag

产品编号

KMP4476

别名

甲型流感病毒H9N2血凝素蛋白1, H9N2 HA1, Hemagglutinin Protein, H9N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H9N2血凝素蛋白1, H9N2 HA1, Hemagglutinin Protein, H9N2 HA1 Protein
Catalog Number	KMP4476
Product Description	The H9N2 (A/Hong Kong/1073/99) HA1 Protein(KMP4476) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/Hong Kong/1073/99(H9N2)) hemagglutinin (NP_859037.1) (Met1-Arg335), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H9N2 HA1
Species	H9N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-H9N2 HA1的公共卫生风险如何？

H9N2是禽流感病毒，但其HA1的哺乳动物适应性突变（如
Q226L）可能增加人畜共患风险。
Q2-能否用于疫苗交叉反应性研究？

适用于评估现有H9疫苗对异源毒株（如G1或Y280谱系）的保护效果。
Q3-是否形成三聚体结构？

经负染电镜和SEC-MALS验证，HA1以三聚体形式存在，稳定性良好（Tm≥60℃）。
Q4-如何检测其与抗体的结合？

使用H9N2特异性单抗（如8B4）进行ELISA或SPR检测，KD值≤1 nM。
Q5-推荐动物免疫剂量？

小鼠初次免疫建议20 μg（弗氏佐剂），加强免疫10 μg，间隔3周，共2次。

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