H5N1 (A/turkey/Turkey/1/2005) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H5N1 (A/turkey/Turkey/1/2005) HA Protein, His Tag

H5N1 (A/turkey/Turkey/1/2005) HA Protein, His Tag

产品编号

KMP4470

别名

甲型流感病毒H5N1血凝素蛋白, H5N1 HA, Hemagglutinin Protein, H5N1 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N1血凝素蛋白, H5N1 HA, Hemagglutinin Protein, H5N1 HA Protein
Catalog Number	KMP4470
Product Description	The H5N1 (A/turkey/Turkey/1/2005) HA Protein(KMP4470) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/turkey/Turkey/1/2005(H5N1)) hemagglutinin (Q207Z6, with aa 339-342 mutated from "GERR" to "RESR") (Met1-Gln531), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H5N1 HA
Species	H5N1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, 0.6% Triton X-100, 7% Trehalose, 6% Mannitol, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白的高致病性是否影响实验操作？

本产品为重组蛋白，不含活病毒成分，按BSL-1操作即可，但需遵循当地生物安全法规。
Q2-能否用于广谱中和抗体研究？

适用于筛选靶向HA茎部区（stalk domain）的抗体，评估其对不同H5亚型的交叉保护。
Q3-是否验证其与α2,3受体的结合？

经固相糖芯片分析，HA优先结合禽类α2,3唾液酸受体，效价≥1:2048。
Q4-表达系统及纯度如何？

在昆虫细胞（Sf9）中表达，经Ni柱和分子筛纯化，纯度>95%，内毒素<0.05 EU/μg。
Q5-如何检测蛋白的天然构象？

通过圆二色谱（CD）分析α-螺旋含量，或使用构象特异性单抗（如CR6261）进行验证。

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