H1N1 (A/California/04/2009) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H1N1 (A/California/04/2009) HA Protein, His Tag

H1N1 (A/California/04/2009) HA Protein, His Tag

产品编号

KMP4464

别名

甲型流感病毒H1N1血凝素蛋白, H1N1 HA, Hemagglutinin Protein, H1N1 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H1N1血凝素蛋白, H1N1 HA, Hemagglutinin Protein, H1N1 HA Protein
Catalog Number	KMP4464
Product Description	The H1N1 (A/California/04/2009) HA Protein(KMP4464) is produced in Insect Cells. A DNA sequence encoding the extracellular domain of Influenza A virus (A/California/04/2009 (H1N1)) (ACP41105.1) hemagglutinin (Met1-Gln529) (Native, HA1+HA2, uncleaved) was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Molecular Name	H1N1 HA
Species	H1N1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否包含2009年H1N1大流行株的抗原特征？

是的，包含关键表位（如Sa和Sb位点），模拟A/California/04/2009株的HA结构。
Q2-能否用于季节性流感疫苗研发？

可以，作为灭活疫苗或重组亚单位疫苗的候选抗原，支持临床前研究。
Q3-如何检测HA的受体结合特异性？

通过唾液酸受体结合实验（如α-2,6连接偏好性），验证其与人类呼吸道上皮的结合能力。
Q4-是否提供预包被ELISA板？

支持，提供预包被HA的96孔板，优化封闭条件，直接用于抗体效价检测。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后避免反复冻融。

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