EBV (strain B95-8) gH & gL Heterodimer Protein, His Tag, Flag Tag_天津卡梅德科技发展有限公司

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EBV (strain B95-8) gH & gL Heterodimer Protein, His Tag, Flag Tag

EBV (strain B95-8) gH & gL Heterodimer Protein, His Tag, Flag Tag

产品编号

KMP4463

别名

巴二氏病毒gH和gL的异二聚体蛋白, EBV gH & gL Heterodimer, gH & gL Heterodimer, EBV gH & gL Heterodimer Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	巴二氏病毒gH和gL的异二聚体蛋白, EBV gH & gL Heterodimer, gH & gL Heterodimer, EBV gH & gL Heterodimer Protein
Catalog Number	KMP4463
Product Description	The EBV (strain B95-8) gH & gL Heterodimer Protein(KMP4463) is produced in HEK293 Cells. A DNA sequence encoding the (Ser19-Leu682) of Epstein-Barr virus (EBV) (strain B95-8) gH (P03231) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 , A DNA sequence encoding the (Asn23-Gly137) of Epstein-Barr virus (EBV) (strain B95-8) gL (P03212) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
Molecular Name	EBV gH & gL Heterodimer
Species	EBV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gH/gL异二聚体在EB病毒中的功能是什么？

gH/gL复合体介导病毒与宿主细胞的膜融合，是疫苗和中和抗体开发的关键靶点。
Q2-双标签（His & Flag）的作用是什么？

His标签用于纯化，Flag标签便于检测；Flag可通过肠激酶切割去除（需在订购时注明）。
Q3-能否用于EBV诊断试剂开发？

是的，作为ELISA抗原检测患者血清中的IgG/IgM抗体，特异性＞95%。
Q4-如何验证复合体的正确组装？

通过非还原SDS-PAGE和质谱分析，确认gH/gL以1:1比例稳定结合。
Q5-是否支持动物免疫实验？

支持，提供佐剂（如CpG）配伍建议，已用于兔模型诱导中和抗体。

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