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EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix Protein, His Tag

EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix Protein, His Tag

产品编号

KMP4454

别名

埃博拉病毒基质蛋白, EBOV Matrix, VP40, EBOV Matrix Protein

规格
  • 50ug
  • 100ug
  • 200ug
产品介绍
Alias埃博拉病毒基质蛋白, EBOV Matrix, VP40, EBOV Matrix Protein
Catalog NumberKMP4454
Product DescriptionThe EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix Protein(KMP4454) is produced in E. coli. A DNA sequence encoding the Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP40 (AHX24648.1) (Asn31-Lys326) was expressed with a polyhistidine tag at the N-terminus.
Molecular NameEBOV Matrix
SpeciesEBOV
HostE. coli
Size50ug, 100ug, 200ug
Purity>85% as determined by SDS-PAGE
PurificationAffinity purification
Endotoxin<1.0 EU/ug determined by the LAL method
Formulation20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
BackgroundInfluenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.
FAQ
  • Q1-基质蛋白在埃博拉病毒中的功能是什么?

    基质蛋白(VP40)参与病毒粒子组装和出芽,是病毒形态发生的关键调控因子。

  • Q2-能否用于病毒样颗粒(VLP)构建?

    可以,与GP蛋白共表达形成VLP,模拟天然病毒用于疫苗或药物筛选。

  • Q3-如何验证蛋白的寡聚化能力?

    通过凝胶过滤色谱和负染电镜分析,确认其形成六聚体或丝状结构。

  • Q4-是否支持抗病毒药物研究?

    支持,作为靶标蛋白用于筛选抑制病毒组装的候选化合物。

  • Q5-运输和储存条件是什么?

    液体需-80℃保存,冻干粉可常温运输,复溶后分装避免反复冻融。

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