EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix Protein, His Tag
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产品编号
KMP4454
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别名
埃博拉病毒基质蛋白, EBOV Matrix, VP40, EBOV Matrix Protein
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规格
- 50ug
- 100ug
- 200ug
Alias | 埃博拉病毒基质蛋白, EBOV Matrix, VP40, EBOV Matrix Protein |
Catalog Number | KMP4454 |
Product Description | The EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Matrix Protein(KMP4454) is produced in E. coli. A DNA sequence encoding the Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP40 (AHX24648.1) (Asn31-Lys326) was expressed with a polyhistidine tag at the N-terminus. |
Molecular Name | EBOV Matrix |
Species | EBOV |
Host | E. coli |
Size | 50ug, 100ug, 200ug |
Purity | >85% as determined by SDS-PAGE |
Purification | Affinity purification |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0 |
Background | Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. |
Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
基质蛋白(VP40)参与病毒粒子组装和出芽,是病毒形态发生的关键调控因子。
可以,与GP蛋白共表达形成VLP,模拟天然病毒用于疫苗或药物筛选。
通过凝胶过滤色谱和负染电镜分析,确认其形成六聚体或丝状结构。
支持,作为靶标蛋白用于筛选抑制病毒组装的候选化合物。
液体需-80℃保存,冻干粉可常温运输,复溶后分装避免反复冻融。