Human THPO Protein, His Tag
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产品编号
KMPH1537
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别名
血小板生成素, thrombopoietin, TPO, THPO, MGDF, MKCSF
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规格
- 100ug
- 1mg
产品介绍
| Alias | 血小板生成素, thrombopoietin, TPO, THPO, MGDF, MKCSF |
| Catalog Number | KMPH1537 |
| Molecular Name | thrombopoietin |
| Species | Human |
| Host | HEK293 Cells |
| Size | 100ug, 1mg |
| Purity | > 90% as determined by SDS PAGE |
| Endotoxin | Less than 1 EU/ug as determined by LAL test. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Background | Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action. |
| Application | SDS-PAGE, WB, ELISA |
| Tag | His tag |
| Biological Activity | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-60 ng/ml. |
| Predicted N Terminus | Ser 22 |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Sequence | SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEW KTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLG ALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVR RAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLKWQQGF RAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAP |
| Molecular Weight | The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 78.2 kDa band in SDS-PAGE under reducing conditions. |
| Uniprot ID | P40226 |
| Note | 仅供科研使用 |
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