Human EPO Protein, Fc Tag
-
产品编号
KMPH1383
-
别名
促红细胞生成素, Erythropoietin, EPO, human EPO, human Erythropoietin, EPO Protein, human EPO Protein
-
规格
- 100ug
- 1mg
产品介绍
| Alias | 促红细胞生成素, Erythropoietin, EPO, human EPO, human Erythropoietin, EPO Protein, human EPO Protein |
| Catalog Number | KMPH1383 |
| Molecular Name | Erythropoietin, EPO |
| Species | Human |
| Host | HEK293 Cells |
| Size | 100ug, 1mg |
| Purity | > 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Endotoxin | < 10 EU per mg protein. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Background | Erythropoietin is a member of the EPO / TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. It has a longer circulating half-life in vivo. Erythropoietin is being much misused as a performance-enhancing drug in endurance athletes. |
| Application | SDS-PAGE, WB, ELISA |
| Tag | hFc tag |
| Biological Activity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2.0-8.0 ng/mL. |
| Predicted N Terminus | Ala 28 |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Sequence | MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLEAKEAEN ITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVL RGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAAS AAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR |
| Molecular Weight | The recombinant human EPO consists 404 amino acids and predicts a molecular mass of 45.1 kDa. |
| Uniprot ID | P01588 |
| Note | 仅供科研使用 |
- 联系我们
- 电话：400-621-6806
- 手机：13116038708
- 邮箱：info@kmdbioscience.com
- 地址：天津市武清区国际企业社区A5号楼
-
销售微信
-
官方公众号
-
400-621-6806 -
614353794 -
周一至周五 9:00-18:00 -
-
0
-
