Alpaca IgG1 Native Protein_天津卡梅德科技发展有限公司

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Alpaca IgG1 Native Protein

Alpaca IgG1 Native Protein

产品编号

TKPA108

别名

Alpaca IgG1 Native Protein, IgG1, Alpaca Immunoglobulin Isotype-1, alpaca control antibody, alpaca isotype control

规格

1mg
5mg
10mg

DATASHEET MSDS

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产品介绍

Alias	Alpaca IgG1 Native Protein, IgG1, Alpaca Immunoglobulin Isotype-1, alpaca control antibody, alpaca isotype control
Catalog Number	TKPA108
Host	Alpaca
Size	1mg,5mg,10mg
Clonity	Monoclonal
Purity	>90%
Concentration	1mg/ml
Purification	IEX+Affinity purification
Formulation	10mM Tris+0.1M Gly, pH7.0
Background	Three subclasses of IgG can be isolated from the serum of camelids. Conventional IgG1 contains molecules of ~170KD, which upon reduction yield ~50KD heavy chains and large ~30KD light chains. IgG2 and IgG3 are distinct in that they do not incorporate light chains. The IgG2 contain molecules of ~100KD, which upon reduction yield only heavy chains of ~49KD. The IgG3 contain molecules of ~90KD, which upon reduction yield only heavy chains of ~46KD. Alpaca IgG1 (TKPA108) is an isotype control antibody, which can be used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Tag	Unconjugated
Storage Condition	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	Shipped on ice packs.
Product Declaration	本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。
Subtype	Alpaca IgG1 Isotype control

FAQ

Q1- 该蛋白的天然构象有何应用优势？

天然IgG1保留完整空间构象，适用于免疫原性研究、抗体制备或结构生物学（如X射线晶体学）。
Q2- 蛋白纯度如何验证？

通过HPLC或SDS-PAGE（纯度≥95%）分析，辅以质谱确认分子量（约150 kDa，含重链和轻链）。
Q3- 是否适用于免疫动物产生多克隆抗体？

是的，天然IgG1免疫原性更接近生理状态。
Q4- 与重组IgG1蛋白有何区别？

天然蛋白含完整翻译后修饰（如糖基化），重组蛋白可能缺乏某些修饰，需根据实验目的选择。
Q5- 如何避免蛋白聚集或降解？

使用含0.1% BSA或甘油的PBS缓冲液溶解，分装冻存，避免反复冻融和高温处理。

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