Alpaca IgG3_天津卡梅德科技发展有限公司

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Alpaca IgG3

产品编号

TKPA106

别名

Alpaca IgG3 Native Protein, IgG3, Alpaca Immunoglobulin Isotype-3, alpaca control antibody, alpaca isotype control

规格

1mg
5mg
10mg

DATASHEET MSDS

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产品介绍

Alias	Alpaca IgG3 Native Protein, IgG3, Alpaca Immunoglobulin Isotype-3, alpaca control antibody, alpaca isotype control
Catalog Number	TKPA106
Host	Alpaca
Size	1mg,5mg,10mg
Clonity	Monoclonal
Purity	>90%
Concentration	1mg/ml
Purification	IEX+Affinity purification
Formulation	10mM Tris+0.1M Gly, pH7.0
Background	Three subclasses of IgG can be isolated from the serum of camelids. Conventional IgG1 contains molecules of ~170KD, which upon reduction yield ~50KD heavy chains and large ~30KD light chains. IgG2 and IgG3 are distinct in that they do not incorporate light chains. The IgG2 contain molecules of ~100KD, which upon reduction yield only heavy chains of ~49KD. The IgG3 contain molecules of ~90KD, which upon reduction yield only heavy chains of ~46KD. Alpaca IgG3 (TKPA106) is an isotype control antibody, which can be used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Tag	Unconjugated
Storage Condition	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	Shipped on ice packs.
Product Declaration	本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。
Subtype	IgG3

FAQ

Q1- Alpaca IgG3与IgG2的主要区别是什么？

IgG3的重链结构域与IgG2不同，可能导致抗原结合特性差异；IgG3更易形成二聚体，适用于特定靶点结合研究。
Q2- 该抗体是否适用于噬菌体展示文库构建？

是的，IgG3的VHH区域可通过基因工程构建文库，筛选高亲和力单域抗体。
Q3- 推荐保存条件是什么？

建议分装后-80℃长期保存，避免反复冻融；短期使用可置于-20℃（含稳定剂）。
Q4- 能否用于流式细胞术（Flow Cytometry）？

可以，但需优化封闭条件以减少非特异性结合。
Q5- 是否与其他物种的IgG有交叉反应？

羊驼IgG3与人类或小鼠IgG无交叉反应，但需验证与其他骆驼科动物IgG的交叉性。

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