Alpaca IgG2_天津卡梅德科技发展有限公司

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Alpaca IgG2

产品编号

TKPA105

别名

Alpaca IgG2 Native Protein, IgG2, Alpaca Immunoglobulin Isotype-2, alpaca control antibody, alpaca isotype control

规格

1mg
5mg
10mg

DATASHEET MSDS

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产品介绍

Alias	Alpaca IgG2 Native Protein, IgG2, Alpaca Immunoglobulin Isotype-2, alpaca control antibody, alpaca isotype control
Catalog Number	TKPA105
Host	Alpaca
Size	1mg,5mg,10mg
Clonity	Monoclonal
Purity	>90%
Concentration	1mg/ml
Purification	IEX+Affinity purification
Formulation	10mM Tris+0.15M NaCl, pH7.0
Background	Three subclasses of IgG can be isolated from the serum of camelids. Conventional IgG1 contains molecules of ~170KD, which upon reduction yield ~50KD heavy chains and large ~30KD light chains. IgG2 and IgG3 are distinct in that they do not incorporate light chains. The IgG2 contain molecules of ~100KD, which upon reduction yield only heavy chains of ~49KD. The IgG3 contain molecules of ~90KD, which upon reduction yield only heavy chains of ~46KD. Alpaca IgG2 (TKPA105) is an isotype control antibody, which can be used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Tag	Unconjugated
Storage Condition	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	Shipped on ice packs.
Product Declaration	本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。
Subtype	IgG2

FAQ

Q1- Alpaca IgG2与其他哺乳动物IgG有何不同？

羊驼IgG2仅包含重链（无轻链），分子量更小（约90 kDa），适合单域抗体（VHH）开发，与常规哺乳动物IgG结构差异显著。
Q2- 该抗体适用于哪些实验？

可用于免疫沉淀（IP）、免疫组化（IHC）及单域抗体制备，也可作为抗原用于抗体制剂开发。
Q3- 能否与Protein A/G结合用于纯化？

羊驼IgG2与Protein A结合较弱，推荐使用Llama IgG特异性亲和填料（如Protein L）进行纯化。
Q4- 如何验证IgG2的纯度？

可通过SDS-PAGE（非还原条件）检测单一条带（约90 kDa），或使用ELISA验证特异性。
Q5- 是否可用于免疫动物制备二抗？

可以，但需使用针对羊驼IgG2独特表位的宿主（如兔）制备特异性二抗。

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