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Alpaca Total IgG(IgG1+IgG2+IgG3)

Alpaca Total IgG(IgG1+IgG2+IgG3)

产品编号

TKPA101

别名

Alpaca IgG Native Protein, alpaca control antibody, alpaca isotype control

规格
  • 1mg
  • 5mg
  • 10mg
产品介绍
AliasAlpaca IgG Native Protein, alpaca control antibody, alpaca isotype control
Catalog NumberTKPA101
HostAlpaca
Size1mg,5mg,10mg
ClonityPolyclonal
Purity>90%
Concentration1mg/ml
PurificationIEX+Affinity purification
Formulation150mM Nacl+0.1M Gly, pH7.0
BackgroundThree subclasses of IgG can be isolated from the serum of camelids. Conventional IgG1 contains molecules of ~170KD, which upon reduction yield 50KD heavy chains and large 30KD light chains. IgG2 and IgG3 are distinct in that they do not incorporate light chains. The IgG2 contain molecules of ~100KD, which upon reduction yield only heavy chains of ~49KD. The IgG3 contain molecules of ~90KD, which upon reduction yield only heavy chains of ~46KD. Alpaca Total IgG (TKPA101) is an isotype control antibody, which can be used as negative controls to help differentiate non-specific background signal from specific antibody signal.
TagUnconjugated
Storage ConditionThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionShipped on ice packs.
Product Declaration本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。
SubtypeIgG1,IgG2,IgG3
FAQ
  • Q1- 该产品的主要用途是什么?

    ①纳米抗体(VHH)开发:作为总IgG来源构建噬菌体展示文库,筛选单域抗体。②免疫检测:作为二抗(如HRP标记)检测羊驼来源的一抗,适用于ELISA、Western blot等实验。

  • Q2- 是否包含所有IgG亚型?

    是,包含IgG1、IgG2和IgG3三种亚型,覆盖羊驼IgG的完整群体。

  • Q3- 如何保存?

    ①液体形式:4℃保存6个月(含0.05% NaN₃),长期保存需分装-20℃(含50%甘油)。②冻干粉:-80℃保存5年,复溶后4℃保存1周。

  • Q4- 是否与其他物种IgG交叉反应?

    仅特异性结合羊驼IgG,与人、小鼠、大鼠IgG无交叉反应(经ELISA验证)。

  • Q5- 是否适配噬菌体展示文库构建?

    是,适用于免疫后外周血单核细胞(PBMC)的mRNA提取及VHH文库构建。

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