Rabbit Anti WASH1/WASHC1 Polyclonal Antibody_天津卡梅德科技发展有限公司

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Rabbit Anti WASH1/WASHC1 Polyclonal Antibody

Rabbit Anti WASH1/WASHC1 Polyclonal Antibody

产品编号

PAV4343

别名

WASH1/WASHC1 Antibody, Rabbit PAb, Antigen Affinity Purified, Anti-FAM39E Antibody, Anti-WASH Antibody, WAS protein family homolog 1

规格

20ul
50ul
100ul

DATASHEET MSDS

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产品介绍

Alias	WASH1/WASHC1 Antibody, Rabbit PAb, Antigen Affinity Purified, Anti-FAM39E Antibody, Anti-WASH Antibody, WAS protein family homolog 1
Catalog Number	PAV4343
Product Description	Rabbit Anti-WASH1/WASHC1 Polyclonal Antibody was obtained by affinity purification from a rabbit immunized with a purified synthesized peptide derived from human WASH1.
Host	Rabbit
Size	20ul, 50ul, 100ul
Clonity	Polyclonal
Concentration	1 mg/ml
Purification	Protein A/G
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Background	WASHC1 (WASH1) is a protein coding gene with endocytosis as a related pathway. Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL, proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.
Immunogen	Synthesized peptide derived from human WASH1
Recommended Dilution	1: 500 - 1: 2000
Storage Condition	Store at -20℃. Avoid freeze / thaw cycles.
Shipping Condition	This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Cross-reactivity	Human, Mouse, Rat
Product Declaration	本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。

FAQ

Q1- WASH1蛋白的功能是什么？

WASH1参与调控肌动蛋白细胞骨架组装，影响胞内运输和细胞迁移。
Q2- 该抗体适用的样本类型？

适用于细胞裂解物、组织切片（石蜡/冰冻），已验证用于WB、IHC和免疫荧光。
Q3- 是否识别其他物种（如小鼠）的WASH1蛋白？

需查看序列同源性，若目标物种WASH1与人源同源性高，则可能交叉反应。
Q4- 是否需要抗原修复？

用于IHC时建议柠檬酸缓冲液（pH6.0）热修复以增强信号。
Q5- 抗体稳定性如何？

长期保存于-20℃，避免反复冻融；工作液4℃可保存1周。

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