Bifunctional protein BirA, His & MBP Tag
-
产品编号
PB110
-
别名
-
规格
- 50ug
- 100ug
Catalog Number | PB110 |
Molecular Name | BirA |
Species | E. coil |
Host | E. coli |
Size | 50ug, 100ug |
Purity | >95%, determined by SDS-PAGE |
Formulation | Lyophilized from sterile PBS, pH 7.4, and 0.01% Tween80 are added as protectants |
Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Shipping Condition | Lyophilized powder will be shipped on at ambient temperature. Please contact our customer service to get specific information about the shipping condition of your order. |
具有生物素连接酶活性,可将生物素标记到特定蛋白,同时参与生物素代谢调控。
His标签用于镍柱纯化,MBP标签可增强蛋白可溶性,并辅助通过直链淀粉树脂纯化。
用于生物素化标记实验、蛋白质相互作用研究、体外酶活性分析及结构生物学研究。
建议分装后-80℃保存,避免反复冻融;短期使用可置于-20℃(含50%甘油缓冲液)。
取决于实验需求,MBP/His标签通常不影响酶活性,但若需天然结构可通过蛋白酶切除。